Journal: The Journal of Clinical Investigation

Article Title: Stromal oncostatin M cytokine promotes breast cancer progression by reprogramming the tumor microenvironment

doi: 10.1172/JCI148667

Figure Lengend Snippet: ( A ) Heatmap showing normalized mRNA expression of genes induced by OSM in CAF-173 and included in the indicated Gene Ontology (GO) pathway. ( B ) Gene set enrichment analysis (GSEA) showing enrichment of inflammatory hallmark signature in microarray expression data of CAF-173 spheres treated with 30 ng/mL OSM for 4 days. NES, normalized enrichment score. ( C and D ) Chemokine array analysis ( C ) and VEGF levels ( D ) in conditioned media from CAF-173 treated with PBS or 30 ng/mL OSM for 72 hours. * P < 0.05, ** P < 0.01, *** P < 0.001. P values were determined using paired, 2-tailed Student’s t tests; n = 4 independent experiments.

Article Snippet: A panel of 31 human chemokines was analyzed by Human Chemokine Array Kit (Proteome Profiler Array, R&D Systems), and VEGF levels were quantified by Human VEGF Quantikine ELISA Kit (R&D Systems) following the manufacturer’s instructions.

Techniques: Expressing, Microarray

Journal: Mutation Research. Reviews in Mutation Research

Article Title: Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

doi: 10.1016/j.mrrev.2017.05.001

Figure Lengend Snippet: Comparative Evaluation For Various Versions Of Ligase Chain Reaction (LCR) And Ligation-Based Amplifications .

Article Snippet: 8- MOL-PCR , - Ease of high level of multiplexing (74) - Improved sensitivity (76) - Enhanced specificity - Reduced cost for multiplexing reactions (76) - Robustness and reduced analysis time (74) - High-throughput with full automation - Commonly used with wide applications , - Requires expensive luminex flow cytometry analyzer and the use of xMAP microsphere , - less than 1000 molecules (74) - Detection limit of as low as 0.1 ng of input DNA (76) , - Reagent costs/SNP is estimated to be 0.8 euro in a singleplex reaction and drops down to 0.15 for 8-plex reaction (76). Although reagents are not expensive, high costs are needed once for the Luminex flow cytometry analyzer - Costs could also be reduced significantly by using less beads and universal biotinylated oligonucleotide , - Reading time for a 96 well plate is less than 45 min with quick analysis time in 30 s for multiplexed samples through the use of flow cytometry (74) , - Yes - Detection of 50 SNPs/well in a 96 well plate (77) , - Thermal cycler and Flow cytometry based analyzer (Luminex) and xMAP microsphere beads (74–76) , - Very common , - Flow cytometric detection using xMAP microsphere and analyzer (74–76) , - Detection of plant pathogens such as Citrus tristeza virus (CTV), Xanthomonas genus and Xylella fastidiosa (74,75) - Antibiotic resistant bacteria such as Yersinia pestis, F. tularensis and Bacillus anthracis (74,75) - Mycobacterium tuberculosis complex (MTBC) (76) , - Yes , - No.

Techniques: Ligation, Multiplexing, Produced, Amplification, High Throughput Screening Assay, Spectrophotometry, MicroChIP Assay, Electrophoresis, Autoradiography, Fluorescence, Mutagenesis, Preserving, Microarray, Labeling, Magnetic Beads, Generated, Multiplex Assay, Real-time Polymerase Chain Reaction, Recombinant, RNA Detection, Detection Assay, Multiplex Ligation-dependent Probe Amplification, Sequencing, Hybridization, Transgenic Assay, Methylation, Luminex, Flow Cytometry, Mass Spectrometry, Colorimetric Assay, SPR Assay, Imaging

Journal: Mutation Research. Reviews in Mutation Research

Article Title: Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

doi: 10.1016/j.mrrev.2017.05.001

Figure Lengend Snippet: Comparative Evaluation For Various Versions Of Ligase Chain Reaction (LCR) And Ligation-Based Amplifications .

Article Snippet: 8- MOL-PCR , - Ease of high level of multiplexing (74) - Improved sensitivity (76) - Enhanced specificity - Reduced cost for multiplexing reactions (76) - Robustness and reduced analysis time (74) - High-throughput with full automation - Commonly used with wide applications , - Requires expensive luminex flow cytometry analyzer and the use of xMAP microsphere , - less than 1000 molecules (74) - Detection limit of as low as 0.1 ng of input DNA (76) , - Reagent costs/SNP is estimated to be 0.8 euro in a singleplex reaction and drops down to 0.15 for 8-plex reaction (76). Although reagents are not expensive, high costs are needed once for the Luminex flow cytometry analyzer - Costs could also be reduced significantly by using less beads and universal biotinylated oligonucleotide , - Reading time for a 96 well plate is less than 45 min with quick analysis time in 30 s for multiplexed samples through the use of flow cytometry (74) , - Yes - Detection of 50 SNPs/well in a 96 well plate (77) , - Thermal cycler and Flow cytometry based analyzer (Luminex) and xMAP microsphere beads (74–76) , - Very common , - Flow cytometric detection using xMAP microsphere and analyzer (74–76) , - Detection of plant pathogens such as Citrus tristeza virus (CTV), Xanthomonas genus and Xylella fastidiosa (74,75) - Antibiotic resistant bacteria such as Yersinia pestis, F. tularensis and Bacillus anthracis (74,75) - Mycobacterium tuberculosis complex (MTBC) (76) , - Yes , - No.

Techniques: Ligation, Multiplexing, Produced, Amplification, High Throughput Screening Assay, Spectrophotometry, MicroChIP Assay, Electrophoresis, Autoradiography, Fluorescence, Mutagenesis, Preserving, Microarray, Labeling, Magnetic Beads, Generated, Multiplex Assay, Real-time Polymerase Chain Reaction, Recombinant, RNA Detection, Detection Assay, Multiplex Ligation-dependent Probe Amplification, Sequencing, Hybridization, Transgenic Assay, Methylation, Luminex, Flow Cytometry, Mass Spectrometry, Colorimetric Assay, SPR Assay, Imaging

Journal: Mutation Research. Reviews in Mutation Research

Article Title: Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

doi: 10.1016/j.mrrev.2017.05.001

Figure Lengend Snippet: Comparative Evaluation For Various Versions Of Ligase Chain Reaction (LCR) And Ligation-Based Amplifications .

Article Snippet: 8- MOL-PCR , - Ease of high level of multiplexing (74) - Improved sensitivity (76) - Enhanced specificity - Reduced cost for multiplexing reactions (76) - Robustness and reduced analysis time (74) - High-throughput with full automation - Commonly used with wide applications , - Requires expensive luminex flow cytometry analyzer and the use of xMAP microsphere , - less than 1000 molecules (74) - Detection limit of as low as 0.1 ng of input DNA (76) , - Reagent costs/SNP is estimated to be 0.8 euro in a singleplex reaction and drops down to 0.15 for 8-plex reaction (76). Although reagents are not expensive, high costs are needed once for the Luminex flow cytometry analyzer - Costs could also be reduced significantly by using less beads and universal biotinylated oligonucleotide , - Reading time for a 96 well plate is less than 45 min with quick analysis time in 30 s for multiplexed samples through the use of flow cytometry (74) , - Yes - Detection of 50 SNPs/well in a 96 well plate (77) , - Thermal cycler and Flow cytometry based analyzer (Luminex) and xMAP microsphere beads (74–76) , - Very common , - Flow cytometric detection using xMAP microsphere and analyzer (74–76) , - Detection of plant pathogens such as Citrus tristeza virus (CTV), Xanthomonas genus and Xylella fastidiosa (74,75) - Antibiotic resistant bacteria such as Yersinia pestis, F. tularensis and Bacillus anthracis (74,75) - Mycobacterium tuberculosis complex (MTBC) (76) , - Yes , - No.

Techniques: Ligation, Multiplexing, Produced, Amplification, High Throughput Screening Assay, Spectrophotometry, MicroChIP Assay, Electrophoresis, Autoradiography, Fluorescence, Mutagenesis, Preserving, Microarray, Labeling, Magnetic Beads, Generated, Multiplex Assay, Real-time Polymerase Chain Reaction, Recombinant, RNA Detection, Detection Assay, Multiplex Ligation-dependent Probe Amplification, Sequencing, Hybridization, Transgenic Assay, Methylation, Luminex, Flow Cytometry, Mass Spectrometry, Colorimetric Assay, SPR Assay, Imaging

Journal: Mutation Research. Reviews in Mutation Research

Article Title: Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

doi: 10.1016/j.mrrev.2017.05.001

Figure Lengend Snippet: Comparative Evaluation For Various Versions Of Ligase Chain Reaction (LCR) And Ligation-Based Amplifications .

Article Snippet: 8- MOL-PCR , - Ease of high level of multiplexing (74) - Improved sensitivity (76) - Enhanced specificity - Reduced cost for multiplexing reactions (76) - Robustness and reduced analysis time (74) - High-throughput with full automation - Commonly used with wide applications , - Requires expensive luminex flow cytometry analyzer and the use of xMAP microsphere , - less than 1000 molecules (74) - Detection limit of as low as 0.1 ng of input DNA (76) , - Reagent costs/SNP is estimated to be 0.8 euro in a singleplex reaction and drops down to 0.15 for 8-plex reaction (76). Although reagents are not expensive, high costs are needed once for the Luminex flow cytometry analyzer - Costs could also be reduced significantly by using less beads and universal biotinylated oligonucleotide , - Reading time for a 96 well plate is less than 45 min with quick analysis time in 30 s for multiplexed samples through the use of flow cytometry (74) , - Yes - Detection of 50 SNPs/well in a 96 well plate (77) , - Thermal cycler and Flow cytometry based analyzer (Luminex) and xMAP microsphere beads (74–76) , - Very common , - Flow cytometric detection using xMAP microsphere and analyzer (74–76) , - Detection of plant pathogens such as Citrus tristeza virus (CTV), Xanthomonas genus and Xylella fastidiosa (74,75) - Antibiotic resistant bacteria such as Yersinia pestis, F. tularensis and Bacillus anthracis (74,75) - Mycobacterium tuberculosis complex (MTBC) (76) , - Yes , - No.

Techniques: Ligation, Multiplexing, Produced, Amplification, High Throughput Screening Assay, Spectrophotometry, MicroChIP Assay, Electrophoresis, Autoradiography, Fluorescence, Mutagenesis, Preserving, Microarray, Labeling, Magnetic Beads, Generated, Multiplex Assay, Real-time Polymerase Chain Reaction, Recombinant, RNA Detection, Detection Assay, Multiplex Ligation-dependent Probe Amplification, Sequencing, Hybridization, Transgenic Assay, Methylation, Luminex, Flow Cytometry, Mass Spectrometry, Colorimetric Assay, SPR Assay, Imaging